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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab154461-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.
In vitro complement-dependent cytotoxicity assays:Use at an assay dependent dilution.In vivo depletion of Ly-6G+ cells:Use at an assay dependent dilution.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Ly-6G-PE/CY7 with ab25514 at 0.01 μg/106 cells. A Rat Anti-Mouse CD19-PE was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"