Anti-Ly6g抗体[RB6-8C5] (PE/Cy7 ®) (ab25514)

概述

  • 产品名称
    Anti-Ly6g抗体[RB6-8C5] (PE/Cy7 ®)
    参阅全部 Ly6g 一抗
  • 描述
    大鼠单克隆抗体[RB6-8C5] to Ly6g (PE/Cy7 ®)
  • 宿主
    Rat
  • 偶联物
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • Flow Cyt: C57Bl/6 bone marrow cells.
  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab25514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab154461 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution.

In vivo depletion of Ly-6G+ cells: Use at an assay dependent dilution.

WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

靶标

  • 相关性
    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • 细胞定位
    Attached to the membrane by a GPI-anchor.
  • 数据库链接
  • 别名
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

图片

  • C57Bl/6 bone marrow cells stained with 0.25µg ab25514 (solid line) or 0.25µg PE-Cy7-conjugated rat IgG2b isotype control (dashed line).

文献

This product has been referenced in:
  • Tumpey TM  et al. Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea. J Virol 70:898-904 (1996). Read more (PubMed: 8551629) »
  • Lagasse E & Weissman IL Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods 197:139-50 (1996). Read more (PubMed: 8890901) »

See all 11 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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