Anti-Ly6g抗体[RB6-8C5] (FITC) (ab25024)

概述

  • 产品名称
    Anti-Ly6g抗体[RB6-8C5] (FITC)
    参阅全部 Ly6g 一抗
  • 描述
    大鼠单克隆抗体[RB6-8C5] to Ly6g (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    ab25024 recognises mouse Mouse Ly-6G.
  • 经测试应用
    适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • mouse spleen cells.

性能

应用

Our Abpromise guarantee covers the use of ab25024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性
    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • 细胞定位
    Attached to the membrane by a GPI-anchor.
  • 数据库链接
  • 别名
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

图片

  • Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 0.5µg (right panel), Rat IgG2b (FITC) isotype control at 0.5µg (left panel).

  • BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells.  Large cells were then gated and analyzed on a flow cytometer.

  • Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 at 0.5µg.

  • ab25024 at a 1/100 dilution detecting Ly6g in LPS activated monocytes by Flow Cytometry.
    Gating strategy was for alive cells.

    See Abreview

  • ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry.
    Gating strategy was for monocytes.

    See Abreview

  • ab25024 staining Ly6g in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide and fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/10 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live Monocytes.

    See Abreview

  • ab25024 detecting Ly6g in rat spleenocytes by Flow Cytometry. Spleen tissue was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live macrophages.

    See Abreview

文献

This product has been referenced in:
  • Chen D  et al. Protease-activated receptor 1 activation is necessary for monocyte chemoattractant protein 1-dependent leukocyte recruitment in vivo. J Exp Med 205:1739-46 (2008). Flow Cyt ; Mouse . Read more (PubMed: 18606855) »

See 1 Publication for this product

客户评价及客户问答

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab25024. I would also appreciate if you can confirm some further details: 1) I see in your details that you are using 0.75ul (0.375ug) of antibody fo...

Read More

Thank you for submitting an Abreview for ab25024. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive macrophages
Username

Abcam user community

Verified customer

提交于 Apr 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Specification
Spleen from immune mouse
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 16 2012

Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive monocytes
Username

Abcam user community

Verified customer

提交于 Apr 16 2012

Thank you for your patience.


I have now had the Rat IgG1 (FITC) isotype control added to our catalog, ab128477.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for contacting Abcam regarding ab25024.


I am working to have the appropriate isotype control added to our catalog for you to purchase. I will provide the ordering details as soon as they are available.


I hope t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (LPS activated monocytes)
Specification
LPS activated monocytes
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

提交于 Dec 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (cells from blood)
Specification
cells from blood
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

提交于 Dec 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册