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Our Abpromise guarantee covers the use of ab25024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21-25 kDa.
In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution.In vivo depletion of Ly-6G+ cells: Use at an assay dependent dilution.
BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells. Large cells were then gated and analyzed on a flow cytometer.
Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Mouse Ly-6G/Ly-6C with ab25024 at 1 μg/106 cells. A Rat Anti-Mouse CD19-PE was used as the secondary antibody.