Anti-Ly6g抗体[RB6-8C5] (Cy5 ®) (ab24985)

概述

  • 产品名称
    Anti-Ly6g抗体[RB6-8C5] (Cy5 ®)
    参阅全部 Ly6g 一抗
  • 描述
    大鼠单克隆抗体[RB6-8C5] to Ly6g (Cy5 ®)
  • 偶联物
    Cy5 ®. Ex: 650nm, Em: 667nm
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

性能

应用

Our Abpromise guarantee covers the use of ab24985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IHC-Fr
IP
WB
  • 应用说明
    Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
    Complement-dependent cytotoxicity assays: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
    • 细胞定位
      Attached to the membrane by a GPI-anchor.
    • 数据库链接
    • 别名
      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    文献

    ab24985 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.4% Triton X-100
    Fixative
    Formaldehyde
    Username

    Dr. S Gajavelli

    Verified customer

    提交于 Aug 20 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (lung)
    Specification
    lung
    Permeabilization
    Yes - Triton X-100
    Fixative
    Formalin/PFA-fixed paraffin-embedded sections
    Username

    Abcam user community

    Verified customer

    提交于 May 02 2014

    Thank you very much for your interest in Abcam and our products..

    To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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