Anti-Ly6c抗体[HK1.4] (Phycoerythrin) (ab25572)

概述

  • 产品名称Anti-Ly6c抗体[HK1.4] (Phycoerythrin)
    参阅全部 Ly6c 一抗
  • 描述
    大鼠单克隆抗体[HK1.4] to Ly6c (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性ab25572 recognises Ly6c.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, Celluar Activationmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

性能

应用

Our Abpromise guarantee covers the use of ab25572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.2µg for 106 cells. ab154450-Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Celluar Activation Use at an assay dependent concentration. In vitro activation of T lymphocytes.

靶标

  • 相关性Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • 细胞定位Cell membrane; Lipid-anchor, GPI-anchor.
  • 数据库链接
  • 别名
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

Anti-Ly6c antibody [HK1.4] (Phycoerythrin) 图像

  • Flow cytometry analysis of murine splenocytes, staining Ly6c with ab25572.

    Cells were cultured in Cells were isolated via mechanical disruption of the spleen. The sample was incubated with the primary antibody (1/100 in 2% FCS + 0.5 mM EDTA in PBS) for 30 minutes at 4°C.

    Gating Strategy: lymphocytes

Anti-Ly6c antibody [HK1.4] (Phycoerythrin) (ab25572)参考文献

This product has been referenced in:
  • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23806234) »
  • Ocuin LM  et al. Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. J Leukoc Biol 89:423-32 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21106642) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (splenocytes)
Specification splenocytes
Preparation Cell harvesting/tissue preparation method: mechanical disruption of spleen
Sample buffer: 2% FCS, 0.5 mM EDTA in PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
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提交于 Sep 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"