概述

  • 产品名称
    Anti-Ly6c抗体[HK1.4]
    参阅全部 Ly6c 一抗
  • 描述
    大鼠单克隆抗体[HK1.4] to Ly6c
  • 宿主
    Rat
  • 经测试应用
    适用于: Cellular Activation, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    L3 cloned CTL cells

性能

应用

Our Abpromise guarantee covers the use of ab24973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Cellular Activation
Flow Cyt
IHC-Fr
  • 应用说明
    Cellular activation: Use at an assay dependent dilution.
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
    • 细胞定位
      Cell membrane; Lipid-anchor, GPI-anchor.
    • 数据库链接
    • 别名
      • Ly 6c antibody
      • Ly6c protein antibody
      • Ly6c1 antibody
      • Ly6c2 antibody
      • Lymphocyte antigen 6 complex antibody
      • Lymphocyte antigen 6 complex locus C antibody
      • Lymphocyte antigen 6C antibody
      • Lymphocyte antigen 6C1 antibody
      • Lymphocyte antigen 6C2 antibody
      • Lymphocyte antigen Ly 6C antibody
      see all

    图片

    • Bone marrow cells from C57BL/6 mice were labeled with ab24973 and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.

    文献

    This product has been referenced in:
    • Liyanage SE  et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res 151:160-70 (2016). IHC-P . Read more (PubMed: 27544307) »
    • Zhao C  et al. Nuclear Factor E2-related Factor-2 (Nrf2) Is Required for NLRP3 and AIM2 Inflammasome Activation. J Biol Chem 289:17020-17029 (2014). Flow Cyt . Read more (PubMed: 24798340) »

    See all 3 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target retrival high ph
    Permeabilization
    No
    Specification
    mouse spleen
    Blocking step
    Peroxidase block as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Ms. Ines Kleiber-Schaaf

    Verified customer

    提交于 May 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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