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Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
Database link: P0CW02
ab15627 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS. ab15627 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. ab15674 also detects a wide range of endothelial cells.
Our Abpromise guarantee covers the use of ab15627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
ab15627 staining Ly6c in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by proteinase K pretreatment. Samples were incubated with primary antibody (1/400).
ab15627 staining Ly6c in Mouse tumor stroma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeablizied with Tween-20 and blocked with 1% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate pH6. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. A Avidin / Streptovidin amplification kit was used followed by DAB development.
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