This antibody is specific to an epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants. The antibody stains ~ 90% of NK1.1+ CD3 splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice. The antibody also stains 30-80% of NK1.1+ CD3 splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells.
Ly49C, Ly49F, Ly49H and Ly49I are members of the lectin like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down regulated. These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells.