ab93505 reacts with a subset of mouse Ly49 receptors and recognizes a majority of mouse NK cells. It reacts with 3 putative inhibitory members of the Ly49 family, F, I, and C and one putative activating receptor, Ly49H. It does not react with Ly49A, B, D, or G.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25µg for 105 cells.
Use 0.25µg for 105 to 108 cells in a final volume of 100µl.
ab18486-Syrian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Ly49C, Ly49F, Ly49H and Ly49I are members of the lectin like homodimeric Ly49 family of cell surface receptors. Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down regulated. These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells.