概述

  • 产品名称LY 294002
  • 描述
    PI3-kinase inhibitor
  • 生物学描述A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).
  • 纯度> 99%

性能

    LY 294002 图像

    • Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

      Lane 1: Marker
      Lane 2: A431 lysate starved
      Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
      Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
      Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

      Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

      Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

      Observed bands:
      RSK1p90 (pS380) band size: 83 kDa
      AKT1 (pS473) band size: 56 kDa
      ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
      RPS6 (pS235/236) band size: 31 kDa
      Rab11a band size: 24 kDa
      Unknown band: 130kDa

    LY 294002 (ab120243)参考文献

    This product has been referenced in:
    • Gharbi SI  et al. Exploring the specificity of the PI3K family inhibitor LY294002. Biochem J 404:15-21 (2007). Read more (PubMed: 17302559) »
    • Walker EH  et al. Structural determinants of phosphoinositide 3-kinase inhibition by wortmannin, LY294002, quercetin, myricetin, and staurosporine. Mol Cell 6:909-19 (2000). Read more (PubMed: 11090628) »
    • Vlahos CJ  et al. A specific inhibitor of phosphatidylinositol 3-kinase, 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002). J Biol Chem 269:5241-8 (1994). Read more (PubMed: 8106507) »

    See all 5 Publications for this product

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