Anti-LXR alpha + LXR beta抗体(ab24362)

概述

  • 产品名称Anti-LXR alpha + LXR beta抗体
    参阅全部 LXR alpha + LXR beta 一抗
  • 描述
    山羊多克隆抗体to LXR alpha + LXR beta
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human
    预测可用于: Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish
  • 免疫原

    Peptide with sequence (C)RLQDKKLPPLLSEI, from the C-terminus of the human protein sequences according to NP_005684; NP_009052.

  • 阳性对照
    • Human Spleen lysate.

性能

应用

Our Abpromise guarantee covers the use of ab24362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

靶标

  • 相关性LXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Liver X receptor alpha antibody
    • Liver X receptor beta antibody
    • LXR a antibody
    • LXR b antibody
    • LXRalpha antibody
    • LXRbeta antibody
    • NR1H2 antibody
    • NR1H3 antibody
    • Nuclear orphan receptor LXR alpha antibody
    • Nuclear orphan receptor LXR beta antibody
    • Nuclear receptor subfamily 1 group H member 2 antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR beta antibody
    see all

Anti-LXR alpha + LXR beta antibody 图像

  • Anti-LXR alpha + LXR beta antibody (ab24362) at 1 µg/ml + Human spleen lysate(in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hour

Anti-LXR alpha + LXR beta antibody (ab24362)参考文献

This product has been referenced in:
  • Cruz-Garcia L & Schlegel A Lxr-driven enterocyte lipid droplet formation delays transport of ingested lipids. J Lipid Res 55:1944-58 (2014). Read more (PubMed: 25030662) »
  • Manna PR  et al. Mechanisms of action of hormone-sensitive lipase in mouse Leydig cells: its role in the regulation of the steroidogenic acute regulatory protein. J Biol Chem 288:8505-18 (2013). WB ; Mouse . Read more (PubMed: 23362264) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 40 µg
Specification heart
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 06 2012

I am very pleased to hear you would like to accept our offer and test ab24362 in rabbit. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrabbit in WBand include this code in t...

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Application Western blot
Sample Cow Cell lysate - whole cell (muscle cells)
Loading amount 40 µg
Specification muscle cells
Treatment with LXR agonist for 24 hours
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. GOSSELET Fabien

Verified customer

提交于 Nov 29 2010

Thank you for your enquiry. We have the following antibodies which may be of interest to you, please take a look at the online datasheets to see if there are applicable for your experiments (both have been tested in Western blotting): ab3585 - Rabb...

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