重组Anti-LXR alpha抗体[EPR6508(N)] (ab176323)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6508(N)] to LXR alpha
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LXR alpha抗体[EPR6508(N)]
参阅全部 LXR alpha 一抗 -
描述
兔单克隆抗体[EPR6508(N)] to LXR alpha -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep -
免疫原
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阳性对照
- WB: Jurkat, SH-SY5Y, IMR-32 cell non-nuclear and nuclear fractions, HEK 293 transfected with blank vectors, blank vectors non-nuclear fraction and blank vectors nuclear fraction, HEK 293 transfected with His tagged human LXR alpha full length expression vectors non-nuclear fraction and nuclear fraction, HEK 293 transfected with His tagged human LXR alpha and beta full length expression vectors.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6508(N) -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176323于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa. |
靶标
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功能
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
组织特异性
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
- SwissProt: Q62685 Rat
- Unigene: 438863 Human
see all -
别名
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
图片
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All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) cells non-nuclear fraction
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) cells nuclear fraction
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) cells non-nuclear fraction
Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) cells nuclear fraction
Lane 5 : IMR-32 (Human neuroblastoma neuroblast) cells non-nuclear fraction
Lane 6 : IMR-32 (Human neuroblastoma neuroblast) cells nuclear fraction
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 7 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.
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All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution
Lane 1 : HEK 293 transfected with blank vectors, whole cell lysate
Lane 2 : HEK 293 transfected with blank vectors, non-nuclear fraction
Lane 3 : HEK 293 transfected with blank vectors, nuclear fraction
Lane 4 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, non-nuclear fraction
Lane 5 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, nuclear fraction
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 10 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.
ab213204, ab176842 and ab181602 were used as loading controls.
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All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution
Lane 1 : HEK 293 transfected with blank vectors, whole cell lysate
Lane 2 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, whole cell lysate
Lane 3 : HEK 293 transfected with His tagged human LXR beta full length expression vectors, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Ab176323 does not cross activate with human LXR beta.
ab213204 was used as loading control.
数据表及文件
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SDS download
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Datasheet download
文献 (47)
ab176323 被引用在 47 文献中.
- Hori M et al. Acute Cholesterol-Lowering Effect of Exendin-4 in Ldlr-/- and C57BL/6J Mice. J Atheroscler Thromb 30:74-86 (2023). PubMed: 35314564
- Liu B et al. Inhibition of Human Cytomegalovirus Particle Maturation by Activation of Liver X Receptor. Front Microbiol 13:846386 (2022). PubMed: 35330771
- Zhao G et al. Hydrolyzed Bound Phenolics from Rice Bran Alleviate Hyperlipidemia and Improve Gut Microbiota Dysbiosis in High-Fat-Diet Fed Mice. Nutrients 14:N/A (2022). PubMed: 35334934
- Chen L et al. Loss of mitochondrial ATPase ATAD3A contributes to nonalcoholic fatty liver disease through accumulation of lipids and damaged mitochondria. J Biol Chem 298:102008 (2022). PubMed: 35513069
- Qiu R et al. Ginsenosides Rg1 and CK Control Temozolomide Resistance in Glioblastoma Cells by Modulating Cholesterol Efflux and Lipid Raft Distribution. Evid Based Complement Alternat Med 2022:1897508 (2022). PubMed: 36276866