Anti-LXR alpha抗体(ab106464)

概述

  • 产品名称Anti-LXR alpha抗体
    参阅全部 LXR alpha 一抗
  • 描述
    兔多克隆抗体to LXR alpha
  • 特异性At leastthree isoforms of LXR alpha are known to exist; ab106464 will recognize all three. ab106464 is predicted to not crossreact with LXR beta.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 15 amino acids from near the N terminal end of Human LXR alpha (NP_005684).

  • 阳性对照
    • Rat liver tissue and tissue lysate.

应用

Our Abpromise guarantee covers the use of ab106464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • 组织特异性Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Anti-LXR alpha antibody 图像

  • Immunofluorescence of LXR alpha in rat liver tissue with ab106464 at 20 ug/mL.

  • Immunohistochemistry of rat liver tissue with ab106464 at 5 μg/mL

  • Lane 1 : Anti-LXR alpha antibody (ab106464) at 1 µg/ml
    Lane 2 : Anti-LXR alpha antibody (ab106464) at 2 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 50 kDa

Anti-LXR alpha antibody (ab106464)参考文献

ab106464 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (rat liver tissue lysate)
Loading amount 140 µg
Specification rat liver tissue lysate
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2012

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