Lunatic Fringe overexpression 293T裂解物(whole cell) (ab94182)
概述
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产品名称
Lunatic Fringe overexpression 293T裂解物(whole cell) -
常规说明
ab94182 is a 293T cell transfected lysate in which Human Lunatic Fringe has been transiently over-expressed using a pCMV-Lunatic Fringe plasmid. The lysate is provided in 1 x Sample Buffer.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning. Disease: Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3) [MIM:609813]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. Similarity: Belongs to the glycosyltransferase 31 family. PTM: A soluble form may be derived from the membrane form by proteolytic processing.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94182于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94182 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Lunatic Fringe antibody (ab58078) at 1/500 dilution
Lane 1 :Lunatic Fringe overexpression 293T lysate (whole cell) (ab94182)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94182 尚未被引用在任何文献中。