Luminescent ATP Detection Assay试剂盒(ab113849)

概述

  • 产品名称Luminescent ATP Detection Assay试剂盒
    参阅全部 ATP 试剂盒
  • 检测方法Luminescent
  • 检测类型Quantitative
  • 检测时间
    0h 30m
  • 实验步骤Multiple steps standard assay
  • 产品概述

    Luminescent ATP Detection Assay Kit (ab113849) irreversibly inactivates ATP degrading enzymes (ATPases) during the lysis step, ensuring that the luminescent signal obtained truly corresponds to the endogenous levels of ATP. 

     

     

     

  • 说明

    Total levels of cellular ATP can be used to assess cell viability, cell proliferation and cytotoxicity of a wide range of compounds and biological response modifiers.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Reagents

性能

  • 别名
    • Adenosine 5' triphosphate

相关产品

应用

Our Abpromise guarantee covers the use of ab113849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Luminescent ATP Detection Assay Kit 图像

  • The ATP standard curve was prepared as described in the protocol. Background-subtracted data values (mean +/- SD) are graphed.

  • ab113849 ATP detection kit cytotoxicity data. 25000 HepG2 cells were seeded into each well, allowed to adhere and treated for 4 hours with 25µM rotenone and vehicle control (DMSO) in glucose based complete media. After treatment, cells were lysed, exposed to the ATP substrate solution and signal was measured on a luminescent counter. Mean and standard deviation is plotted for 3 replicates from each condition. Rotenone induces cytotoxicity in HepG2 cells.
  • Cellular Energy Flux for HepG2 cells (seeded at 65,000 per well), treated with a combination of drug compounds modulating the ETC (Antimycin A [1 µM] and FCCP [2.5 µM]), shown as a percentage relative to untreated control cells. Comparative measurements were taken with Extracellular Oxygen Consumption Assay (ab197243) (white column) and Glycolysis Assay [Extracellular acidification] (ab197244) (black column) show the shift between mitochondrial respiration and glycolysis and the cellular control of energy (ATP; measured 1h post-treatment using Luminescent ATP Detection Assay kit (ab113849) (striped column)).

实验方案

Luminescent ATP Detection Assay Kit (ab113849)参考文献

This product has been referenced in:
  • Zhou X  et al. 1,25-Dihydroxyvitamin D inhibits glutamine metabolism in Harvey-ras transformed MCF10A human breast epithelial cell. J Steroid Biochem Mol Biol 163:147-56 (2016). Read more (PubMed: 27154413) »
  • Wiens KE & Ernst JD The Mechanism for Type I Interferon Induction by Mycobacterium tuberculosis is Bacterial Strain-Dependent. PLoS Pathog 12:e1005809 (2016). Read more (PubMed: 27500737) »

See all 7 Publications for this product

Product Wall

Measurement of extracellular ATP levels

Good Excellent 5/5 (Ease of Use)
Abreviews
I enrolled to an Abcam trial to check the suitability of this ATP luminescence kit (ab113849) to detect extracellular ATP levels in cancer cells.

I personally recommend this kit to measure extracellular ATP levels for its ease of use and for the kindly attention and help I received from the Abcam scientific support specialists. When measuring extracellular ATP levels, it is important to avoid light exposure as much as possible and measure the supernatant without FBS and without phenol-red.

Brief description of the protocol:
I seeded 50.000 murine breast-to-brain cancer cells in duplicates or triplicates in 96-well-plate. Once they are attached I starve them in FBS-free and phenol red-free media.

24 hours after starving I collected the supernatant (100 ul) and transfer it to a white opaque plate. There I followed the protocol proposed in the booklet with only one difference: I do not use detergent.

Using the same conditions (cell line, passage, starving time and machine) I repeated 3 times with the same cancer cell line and the results I got are 12,89 nM ATP, 12,30 nM ATP and 7,3 nM ATP.

Regarding the conditions I use, I repeated this procedure several times and I chose this condition (24-hours starvation in phenol red-free and serum-free media) because if I starve them for less hours the luminescence units I get from my samples are super low. If I starve them with HBBS overnight the cells look ugly the following day. Moreover I also tried to add detergent in the media but then the RLU I got were more variable in the same biological replicate. There is also higher variability of RLU if I use media with FBS. I did not try to use media with phenol red because I know it interferes with the luminescence readout. To sum up, I got less variability when I starve them for 24 hours with DMEM without FBS and without phenol red.
Username

Lisa Sevenich

Verified customer

提交于 Jul 07 2017

We haven’t addressed this question experimentally with the reagents.  The answer is probably.  The lysis reagent is denaturing so bound ATP may be made available to the luciferase. This would be difficult to be definitive and different ATP binding prot...

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In theory it should work, but we don’t know for sure as we have not tested it. Therefore, if you are willing to test and publish the data in the form of review on our website then I would like to invite you to our Abtrial programme.

Thank you for your enquiry.

Luminescence does not have multiple wavelengths. In Luminescence mode, the instrument simply measures the emission of visible light from the well. What is key in the measurement is the PMT (photomultiplier time). Th...

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Thank you for contacting us.



The cells do not need to rest or adhere for this assay, you can measure right away.



Any anionic detergent will inhibit the luciferase reaction. What is interesting is that non-ionic detergents have the opposite effect and can in fact enhance the luminescent signal. The following detergents used between 0.5 and 1.5% can enhance the sig...

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SDS is a very strong inhibitor of the luciferase reaction. The lowest possible concentration will likely be below 0.05%. We suggest the customer running a standard ATP curve with and w/o SDS to determine empirically the effect.

Theoretically,...

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The mammalian cell lysis solution contains 0.1M of alkaline solution. I don't think it will be compatible with protein quantification. Indeed, changes in ATP may be due to unevenness in seeding or low viability. Using multiple replicates per each dat...

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I contacted the laboratory to have more information about the standardization of the results obtained with the ab113849 but unfortunately the lab does not have this kind of information. I am very sorry for this inconvenience.


However, fro...

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I have looked into this further and can confirm that in testing clear plates have been used with no major issues. However if you do have black plates, that may be more optimal.

1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"