Anti-LTV1抗体(ab122100)

概述

  • 产品名称
    Anti-LTV1抗体
  • 描述
    兔多克隆抗体to LTV1
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LEGSIQVDSN RLQEVLNDYY KEKAENCVKL NTLEPLEDQD LPMNELDESE EEEMITVVLE EAKEKWDCES ICSTYSNLYN HPQLIKYQPK PKQ, corresponding to amino acids of Human LTV1.

  • 阳性对照
    • Human placenta tissue; RT 4, U 251 MG and Human tonsil lysates; U 251MG cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

图片

  • All lanes : Anti-LTV1 antibody (ab122100) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate

    Developed using the ECL technique
  • ab122100, at 1/200, staining LTV1 in paraffin embedded Human placenta tissue by Immunohistochemistry.
  • ab122100, at 4 µg/ml, staining LTV1 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

文献

ab122100 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (neuroblastome)
Specification
neuroblastome
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Username

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提交于 Jan 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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