概述

  • 产品名称Anti-LRWD1抗体
    参阅全部 LRWD1 一抗
  • 描述
    兔多克隆抗体to LRWD1
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 432 and 482 of human LRWD1 (NP_690852.1)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab85738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
    • 序列相似性Belongs to the LRWD1 family.
      Contains 3 LRR (leucine-rich) repeats.
      Contains 5 WD repeats.
    • 结构域The WD repeats are required for the interaction with ORC components.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1200011O22Rik antibody
      • AU042569 antibody
      • AW548074 antibody
      • DKFZp434K1815 antibody
      • Leucine rich repeats and WD repeat domain containing 1 antibody
      • Leucine-rich repeat and WD repeat-containing protein 1 antibody
      • LRWD1 antibody
      • LRWD1_HUMAN antibody
      • ORC-associated protein antibody
      • ORCA antibody
      • Origin recognition complex-associated protein antibody
      see all

    Anti-LRWD1 antibody 图像

    • All lanes : Anti-LRWD1 antibody (ab85738) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 71 kDa
      Observed band size : 71 kDa
      Additional bands at : 50 kDa,80 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    Anti-LRWD1 antibody (ab85738)参考文献

    ab85738 has not yet been referenced specifically in any publications.

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