The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
Is unsuitable for IP.
Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
Belongs to the LRWD1 family. Contains 3 LRR (leucine-rich) repeats. Contains 5 WD repeats.
The WD repeats are required for the interaction with ORC components.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.