• 产品名称Anti-LRWD1抗体
    参阅全部 LRWD1 一抗
  • 描述
    兔多克隆抗体to LRWD1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LRWD1.

  • 阳性对照
    • This antibody gave a positive signal in Human testis tissue lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab107779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).


  • 功能Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
  • 序列相似性Belongs to the LRWD1 family.
    Contains 3 LRR (leucine-rich) repeats.
    Contains 5 WD repeats.
  • 结构域The WD repeats are required for the interaction with ORC components.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1200011O22Rik antibody
    • AU042569 antibody
    • AW548074 antibody
    • DKFZp434K1815 antibody
    • Leucine rich repeats and WD repeat domain containing 1 antibody
    • Leucine-rich repeat and WD repeat-containing protein 1 antibody
    • LRWD1 antibody
    • LRWD1_HUMAN antibody
    • ORC-associated protein antibody
    • ORCA antibody
    • Origin recognition complex-associated protein antibody
    see all

Anti-LRWD1 antibody 图像

  • Anti-LRWD1 antibody (ab107779) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 190 kDa,235 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-LRWD1 antibody (ab107779)参考文献

ab107779 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk McManus

Verified customer

提交于 Jun 01 2012