概述

  • 产品名称Anti-LRTM1抗体
  • 描述
    兔多克隆抗体to LRTM1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to internal sequence amino acids 139-286 of Human LRTM1.

  • 阳性对照
    • Human cerebellum tissue. HEK293T cells ovrexpressing LRTM1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 序列相似性Contains 5 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HT017 antibody
    • Leucine rich repeats and transmembrane domains 1 antibody
    • Leucine-rich repeat and transmembrane domain-containing protein 1 antibody
    • Lrtm1 antibody
    • LRTM1_HUMAN antibody
    see all

Anti-LRTM1 antibody 图像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121409 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121409 at 1/20 dilution staining LRTM1 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • All lanes : Anti-LRTM1 antibody (ab121409) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : HEK293T cells overexpressing LRTM1 (Co-expressed with a C-terminal myc-DDK tag) in mammalian HEK293T cells

    Developed using the ECL technique

Anti-LRTM1 antibody (ab121409)参考文献

ab121409 has not yet been referenced specifically in any publications.

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