antigen sequence, corresponding to internal sequence amino acids 139-286 of Human LRTM1.
- Human cerebellum tissue. HEK293T cells ovrexpressing LRTM1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Anti-LRTM1 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-LRTM1 antibody (ab121409)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121409 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRTM1 antibody (ab121409)
ab121409 at 1/20 dilution staining LRTM1 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
Western blot - Anti-LRTM1 antibody (ab121409)
All lanes : Anti-LRTM1 antibody (ab121409) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : HEK293T cells overexpressing LRTM1 (Co-expressed with a C-terminal myc-DDK tag) in mammalian HEK293T cells
Developed using the ECL technique
Anti-LRTM1 antibody (ab121409)参考文献
has not yet been referenced specifically in any publications.
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