Rat, Cow, Dog, Human, Pig, Macaque monkey, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human LRRTM1.
This antibody gave a positive signal in the following tissue lysates: Mouse Forebrain; Mouse Hippocampus; Mouse Brain P0; Mouse Olfactory Bulb.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
May play a role during the development of specific forebrain structures by influencing neuronal differentiation and connectivity, with a possible role in intracellular trafficking within axons.
Predominantly expression in forebrain regions including thalamus and cerebral cortex.
Belongs to the LRRTM family.
Contains 9 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
Endoplasmic reticulum membrane.
Information by UniProt
Western blot - Anti-LRRTM1 antibody (ab106414)
All lanes :
Anti-LRRTM1 antibody (ab106414) at 1/250 dilution
Lane 1 :
Forebrain (Mouse) Tissue Lysate
Lane 2 :
Mouse Hippocampus Tissue Lysate
Lane 3 : Mouse brain tissue lysate - total protein (0 days) ( ab7188) Lane 4 : Mouse Olfactory Bulb Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa Observed band size: 65 kDa ( why is the actual band size different from the predicted?) Additional bands at: 130 kDa, 30 kDa, 40 kDa. We are unsure as to the identity of these extra bands. Exposure time: 8 minutes LRRTM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
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