Anti-LRRN3抗体(ab82657)

概述

  • 产品名称
    Anti-LRRN3抗体
  • 描述
    兔多克隆抗体to LRRN3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (ELYINHNLLS TISPGAFIGL HNLLRLHLNS NRLQMINSKW FDALPNLEIL) of Human LRRN3 (NP_001093130).

  • 阳性对照
    • Placenta lysate.

性能

应用

Our Abpromise guarantee covers the use of ab82657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.

靶标

图片

  • Anti-LRRN3 antibody (ab82657) at 1 µg/ml (5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    Gel concentration: 12%
  • ab82657 staining LRRN3 on E14.5 mouse embryo (intercostal region) by IHC-Fr (left). The tissue was formaldehyde fixed and then blocked for 1 hour. The primary antibody was diluted 1/300 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary. Nuclei were stained with Hoechst 33342 (right).

    See Abreview

文献

ab82657 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E14.5 wildtype intercostal region)
Specification
E14.5 wildtype intercostal region
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

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提交于 Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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