概述

  • 产品名称Anti-LRRC50抗体
    参阅全部 LRRC50 一抗
  • 描述
    兔多克隆抗体to LRRC50
  • 经测试应用适用于: WB, ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region of Human LRRC50. The immunogen is found within the following sequence which corresponds to aa 151-200 of the human protein: TELRCLFLQMNLLRKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTL .

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab75163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:1562500.

ICC/IF Use at an assay dependent concentration. PubMed: 23599692
IHC-P Use at an assay dependent concentration. PubMed: 23599692

靶标

  • 相关性LRRC50 contains six LRR (leucine-rich) repeats. It is proposed that LRRC50 is a novel candidate gene for human cystic kidney disease, involved in regulation of microtubule-based cilia and actin-based brush border microvilli.
  • 细胞定位Cell projection; cilium. Cytoplasm. Cytoplasm; cytoskeleton, spindle pole. Note: In HEK293T cells, it is diffusely cytoplasmic and concentrates at the mitotic spindle poles, while in MDCK cells, it localizes in the cilium. In vivo, this protein is probably restricted to the cilium.
  • 数据库链接
  • 别名
    • CILD13 antibody
    • DNAAF1 antibody
    • Dynein assembly factor 1, axonemal antibody
    • Leucine rich repeat containing 50 antibody
    • ODA7 antibody
    see all

Anti-LRRC50 antibody 图像

  • Anti-LRRC50 antibody (ab75163) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 80 kDa

Anti-LRRC50 antibody (ab75163)参考文献

This product has been referenced in:
  • Basten SG  et al. Mutations in LRRC50 predispose zebrafish and humans to seminomas. PLoS Genet 9:e1003384 (2013). IHC-P, ICC/IF ; Human, Zebrafish . Read more (PubMed: 23599692) »

See 1 Publication for this product

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