Anti-LRRC47抗体(ab121283)
Key features and details
- Rabbit polyclonal to LRRC47
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LRRC47抗体
参阅全部 LRRC47 一抗 -
描述
兔多克隆抗体to LRRC47 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 348-469 of Human LRRC47 (Q8N1G4).
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阳性对照
- ICC: U-2 OS cells. WB: RT4 and U-251 MG cell lysates. IHC-P: Cerebral cortex, colon, testis and kidney tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121283于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Contains 7 LRR (leucine-rich) repeats. - Information by UniProt
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数据库链接
- Entrez Gene: 57470 Human
- SwissProt: Q8N1G4 Human
- Unigene: 268488 Human
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别名
- 2900010D03Rik antibody
- AA960559 antibody
- Leucine-rich repeat-containing protein 47 antibody
see all
图片
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121283 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-LRRC47 antibody (ab121283) at 0.04 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Performed under reducing conditions.Western blot analysis labeling LRRC47 with ab121283 at 0.04 ug/ml.
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Immunohistochemical analysis of human colon tissue labeling LRRC47 with ab121283 at 0.04 ug/ml.
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Immunohistochemical analysis of human testis tissue labeling LRRC47 with ab121283 at 0.04 ug/ml.
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Immunohistochemical analysis of human kidney tissue labeling LRRC47 with ab121283 at 0.04 ug/ml.
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Immunohistochemical analysis of human cerebral cortex tissue labeling LRRC47 with ab121283 at 0.04 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121283 被引用在 1 文献中.
- Piao JM et al. MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage. Cell Physiol Biochem 46:890-906 (2018). PubMed: 29669322