Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (AEFSATCLNF SGLSLSLPHN QSLRASNVIL LDLSGNGLRE LPVTFFAHLQ) of Human LRRC25 (NP_660299).
- Human fetal brain lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
LRRC25 is a single-pass type I membrane protein. It contains 2 LRR (leucine-rich) repeats. LRRC25 may be involved in the activation of cells of innate and acquired immunity.
Membrane; Single-pass type I membrane protein.
- FLJ38116 antibody
- Leucine rich repeat containing 25 antibody
- Leucine rich repeat containing protein 25 antibody
Western blot - LRRC25 antibody (ab84954)
Anti-LRRC25 antibody (ab84954) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
33 kDaObserved band size:
37 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
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