概述

  • 产品名称Anti-LRPPRC抗体
    参阅全部 LRPPRC 一抗
  • 描述
    兔多克隆抗体to LRPPRC
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 611-907 of Human LRPPRC (NP_573566)

  • 阳性对照
    • H1299, HeLa, 293T, A431, HepG2, Molt-4 and Raji cell lines. SW480 Xenograft.

性能

应用

Our Abpromise guarantee covers the use of ab97505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 158 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.
  • 组织特异性Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.
  • 疾病相关Leigh syndrome French-Canadian type
  • 序列相似性Contains 20 PPR (pentatricopeptide) repeats.
  • 细胞定位Mitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 130 kDa leucine-rich protein antibody
    • gp130 antibody
    • Leucine-rich PPR motif-containing protein antibody
    • Leucine-rich PPR-motif containing protein antibody
    • LPPRC_HUMAN antibody
    • LRP 130 antibody
    • lrpprc antibody
    • mitochondrial antibody
    see all

Anti-LRPPRC antibody 图像

  • All lanes : Anti-LRPPRC antibody (ab97505) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 158 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97505 at 1/200 dilution. Lower panel is also merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using ab97505 at 1/100 dilution.

Anti-LRPPRC antibody (ab97505)参考文献

This product has been referenced in:
  • Edgett BA  et al. The effect of acute and chronic sprint-interval training on LRP130, SIRT3, and PGC-1a expression in human skeletal muscle. Physiol Rep 4:N/A (2016). WB . Read more (PubMed: 27604398) »
  • Clemente P  et al. hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria. J Biol Chem 288:8321-31 (2013). Read more (PubMed: 23362268) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions Reduced Denaturing (6% Gel)
Loading amount 3.25 µg
Specification Skeletal Muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Nov 26 2015

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