概述

  • 产品名称Anti-LRPAP1抗体
    参阅全部 LRPAP1 一抗
  • 描述
    兔多克隆抗体to LRPAP1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human LRPAP1.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HEK293 Whole Cell; A498 Whole Cell; Human Brain Tissue; U-87 MG Whole Cell.

性能

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应用

Our Abpromise guarantee covers the use of ab85591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
  • 疾病相关Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
  • 序列相似性Belongs to the alpha-2-MRAP family.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 39 kDa receptor-associated protein antibody
    • A2MRAP antibody
    • A2RAP antibody
    • Alpha 2 macroglobulin receptor associated protein antibody
    • Alpha 2 MRAP antibody
    • Alpha-2-macroglobulin receptor-associated protein antibody
    • Alpha-2-MRAP antibody
    • AMRP_HUMAN antibody
    • HBP44 antibody
    • Lipoprotein receptor associated protein antibody
    • Low density lipoprotein receptor related protein associated protein 1 antibody
    • Low density lipoprotein receptor-related protein-associated protein 1 antibody
    • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
    • Low density lipoprotein related protein associated protein 1 antibody
    • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
    • Lrpap1 antibody
    • MGC138272 antibody
    • MRAP antibody
    • MYP23 antibody
    • RAP antibody
    see all

Anti-LRPAP1 antibody 图像

  • All lanes : Anti-LRPAP1 antibody (ab85591) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 37 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab85591 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85591, µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa cells at 5µg/ml.
  • IHC image of LRPAP1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85591, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-LRPAP1 antibody (ab85591)参考文献

ab85591 has not yet been referenced specifically in any publications.

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