概述

  • 产品名称Anti-LRP6抗体
    参阅全部 LRP6 一抗
  • 描述
    山羊多克隆抗体to LRP6
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CMTSVATAKGYTSDL

    , corresponding to amino acids 1546-1560 of Human LRP6

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
  • 组织特异性Widely co-expressed with LRP5 during embryogenesis and in adult tissues.
  • 疾病相关Defects in LRP6 are the cause of autosomal dominant coronary artery disease type 2 (ADCAD2) [MIM:610947].
  • 序列相似性Belongs to the LDLR family.
    Contains 4 EGF-like domains.
    Contains 3 LDL-receptor class A domains.
    Contains 20 LDL-receptor class B repeats.
  • 结构域The YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex. The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1.
    The PPPSP motifs play a central role in signal transduction by being phosphorylated, leading to activate the Wnt signaling pathway.
  • 翻译后修饰Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.
    Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.
    Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.
    Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum.
    N-glycosylation is required for cell surface location.
  • 细胞定位Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCAD2 antibody
    • C030016K15Rik antibody
    • Cd antibody
    • FLJ90062 antibody
    • FLJ90421 antibody
    • Low density lipoprotein receptor related protein 6 antibody
    • Low-density lipoprotein receptor-related protein 6 antibody
    • LRP-6 antibody
    • LRP6 antibody
    • LRP6_HUMAN antibody
    • OTTHUMP00000238979 antibody
    • OTTHUMP00000238980 antibody
    • OTTHUMP00000238982 antibody
    see all

Anti-LRP6 antibody 图像

  • Ab24386 (5ug/ml) staining human LRP6 in human liver tissue by immunohistochemistry using paraffin embedded tissue.

    Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    In paraffin embedded Human Liver shows strong cytoplasmic staining in hepatocytes and cytoplasmic staining in bile ductulus cells.
  • ab24386 staining LRP6 in Human Kidney at 4µg/ml by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were subjected to steamed antigen retrieval with citrate buffer pH 9.0, and antibody was visualised with HRP-staining.

Anti-LRP6 antibody (ab24386)参考文献

This product has been referenced in:
  • Tahir SA  et al. Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer. Cancer Res 73:1900-11 (2013). Human . Read more (PubMed: 23302227) »
  • Tawk M  et al. Wnt/beta-catenin signaling is an essential and direct driver of myelin gene expression and myelinogenesis. J Neurosci 31:3729-42 (2011). Read more (PubMed: 21389228) »

See all 2 Publications for this product

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