概述

  • 产品名称Anti-LRP6抗体[1C10]
    参阅全部 LRP6 一抗
  • 描述
    小鼠单克隆抗体[1C10] to LRP6
  • 特异性This antibody shows no cross reactivity with LRP5.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Cytoplasmic domain of human LRP6.

性能

应用

Our Abpromise guarantee covers the use of ab75358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 180 kDa (predicted molecular weight: 179 kDa).
IP 1/1000 - 1/2000.

靶标

  • 功能Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
  • 组织特异性Widely co-expressed with LRP5 during embryogenesis and in adult tissues.
  • 疾病相关Defects in LRP6 are the cause of autosomal dominant coronary artery disease type 2 (ADCAD2) [MIM:610947].
  • 序列相似性Belongs to the LDLR family.
    Contains 4 EGF-like domains.
    Contains 3 LDL-receptor class A domains.
    Contains 20 LDL-receptor class B repeats.
  • 结构域The YWTD-EGF-like domains 1 and 2 are required for the interaction with Wnt-frizzled complex. The YWTD-EGF-like domains 3 and 4 are required for the interaction with DKK1.
    The PPPSP motifs play a central role in signal transduction by being phosphorylated, leading to activate the Wnt signaling pathway.
  • 翻译后修饰Dual phosphorylation of cytoplasmic PPPSP motifs sequentially by GSK3 and CK1 is required for AXIN1-binding, and subsequent stabilization and activation of beta-catenin via preventing GSK3-mediated phosphorylation of beta-catenin. Phosphorylated, in vitro, by GRK5/6 within and outside the PPPSP motifs. Phosphorylation at Ser-1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. Phosphorylation by GSK3B is induced by RPSO1 binding and inhibited by DKK1. Phosphorylated, in vitro, by casein kinase I on Thr-1479.
    Undergoes gamma-secretase-dependent regulated intramembrane proteolysis (RIP). The extracellular domain is first released by shedding, and then, through the action of gamma-secretase, the intracellular domain (ICD) is released into the cytoplasm where it is free to bind to GSK3B and to activate canonical Wnt signaling.
    Palmitoylation on the two sites near the transmembrane domain leads to release of LRP6 from the endoplasmic reticulum.
    Mono-ubiquitinated which retains LRP6 in the endoplasmic reticulum.
    N-glycosylation is required for cell surface location.
  • 细胞定位Membrane. Endoplasmic reticulum. On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation. Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6-signalsomes. Chaperoned to the plasma membrane by MESD.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCAD2 antibody
    • C030016K15Rik antibody
    • Cd antibody
    • FLJ90062 antibody
    • FLJ90421 antibody
    • Low density lipoprotein receptor related protein 6 antibody
    • Low-density lipoprotein receptor-related protein 6 antibody
    • LRP-6 antibody
    • LRP6 antibody
    • LRP6_HUMAN antibody
    • OTTHUMP00000238979 antibody
    • OTTHUMP00000238980 antibody
    • OTTHUMP00000238982 antibody
    see all

Anti-LRP6 antibody [1C10] 图像



  • Predicted band size : 179 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: LRP6 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab75358 observed at 200, 220 kDa. Red - loading control, ab18251, observed at 52 kDa.
    ab75358 was shown to specifically react with LRP6 when LRP6 knockout samples were used. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab75358 and ab18251 (loading control to alpha Tubulin) were diluted at 1/1000  and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 179 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Western blot (100ug) and immunoprecipitation (2mg) analysis of total cell lysates from 293T cells using ab75358.

Anti-LRP6 antibody [1C10] (ab75358)参考文献

This product has been referenced in:
  • Liu CC  et al. Deficiency in LRP6-Mediated Wnt Signaling Contributes to Synaptic Abnormalities and Amyloid Pathology in Alzheimer's Disease. Neuron N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 25242217) »
  • Du C  et al. MiR-126-3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2. J Transl Med 12:259 (2014). Human . Read more (PubMed: 25240815) »

See all 5 Publications for this product

Product Wall

Thank you for your inquiry. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed your protocol information and also checked for other problems reported with this antibody. We have not received many rep...

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Thank you for your inquiry. I can confirm that ab75358 (Anti-LRP6 antibody [1C10]) will bind to the intracellular region of LRP6. The immunogen for this antibody was the cytoplasmic domain of human LRP6. Unfortunately, this mono...

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Thank you for your phone call yesterday and for your patience while I have looked into your question. I have heard back from the lab, and I was told that the LRP6 mab [1C10] has been tested against denatured LRP6 by western blotting and non-denature...

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