amino acids 198-251 (VIIDQLPDLM GLKAVNVDKV VGTNPHADRN GGAATCASSR PTQPGLAAPS RAWN) of Human LRP5L (A4QPB2).
- HEK293T transfected lysate.
Human skeletal muscle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 5 LDL-receptor class B repeats.
Information by UniProt
- Low density lipoprotein receptor-related protein 5 like antibody
- Low-density lipoprotein receptor-related protein 5-like protein antibody
- LRP-5-like antibody
Immunocytochemistry/ Immunofluorescence - Anti-LRP5L antibody (ab122013)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122013 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-LRP5L antibody (ab122013)
All lanes : Anti-LRP5L antibody (ab122013) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : LRP5L (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate
Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP5L antibody (ab122013)
ab122013, at a 1/10 dilution, staining Human LRP5L in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed parraffin-embedded tissue. Moderate cytoplasmic positivity in myocytes shown.
has not yet been referenced specifically in any publications.
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