概述

  • 产品名称Anti-LRP5L抗体
    参阅全部 LRP5L 一抗
  • 描述
    兔多克隆抗体to LRP5L
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    amino acids 198-251 (VIIDQLPDLM GLKAVNVDKV VGTNPHADRN GGAATCASSR PTQPGLAAPS RAWN) of Human LRP5L (A4QPB2).

  • 阳性对照
    • HEK293T transfected lysate. Human skeletal muscle tissue.

性能

应用

Our Abpromise guarantee covers the use of ab122013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-LRP5L antibody 图像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122013 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-LRP5L antibody (ab122013) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : LRP5L (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate

    Developed using the ECL technique
  • ab122013, at a 1/10 dilution, staining Human LRP5L in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed parraffin-embedded tissue. Moderate cytoplasmic positivity in myocytes shown.

Anti-LRP5L antibody (ab122013)参考文献

ab122013 has not yet been referenced specifically in any publications.

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