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Our Abpromise guarantee covers the use of ab36121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|WB||1/200. Predicted molecular weight: 179 kDa. The band seen in the Abreview corresponds to the rabbit protein predicted to by 56kDa|
|IP||Use at an assay dependent concentration. PubMed: 21411632|
ab36121 at 1ug/ml staining LRP5 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9). HRP-staining procedure was used for detection.