Anti-LRP4抗体(ab85697)
Key features and details
- Goat polyclonal to LRP4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LRP4抗体
参阅全部 LRP4 一抗 -
描述
山羊多克隆抗体to LRP4 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide:
C-SNPSYRTSTQEVK
, corresponding to internal sequence amino acids 1765-1777 of Human LRP4 (NP_002325.2). -
阳性对照
- Human brain (cerebral cortex) lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab85697 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. -
组织特异性
Expressed in several regions of the brain. -
疾病相关
Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. -
序列相似性
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 504317 Cow
- Entrez Gene: 4038 Human
- Entrez Gene: 228357 Mouse
- Entrez Gene: 83469 Rat
- Omim: 604270 Human
- SwissProt: O75096 Human
- SwissProt: Q8VI56 Mouse
- SwissProt: Q9Z319 Mouse
see all -
别名
- KIAA0816 antibody
- LDLR dan antibody
- Low density lipoprotein receptor related protein 4 antibody
see all
图片
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Anti-LRP4 antibody (ab85697) at 0.1 µg/ml + Human cerebral cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 216 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
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Datasheet download
文献 (3)
ab85697 被引用在 3 文献中.
- Ohkawara B et al. CTGF/CCN2 facilitates LRP4-mediated formation of the embryonic neuromuscular junction. EMBO Rep 21:e48462 (2020). PubMed: 32558157
- Selcen D et al. Impaired Synaptic Development, Maintenance, and Neuromuscular Transmission in LRP4-Related Myasthenia. JAMA Neurol 72:889-96 (2015). PubMed: 26052878
- Ohkawara B et al. LRP4 third ß-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet N/A:N/A (2013). PubMed: 24234652