- 数据表
- 具体的参考文献 (14)
- 实验方案
概述
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产品名称Anti-LRP1抗体[5A6]
参阅全部 LRP1 一抗 -
描述小鼠单克隆抗体[5A6] to LRP1
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经测试应用适用于: Flow Cyt, WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
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种属反应性与反应: Mouse, Rat, Rabbit, Human
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免疫原
Recombinant fragment (Human), 85kDa cleaved fragment of Low Density LRP
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阳性对照
- Ab28320 gave a positive signal in Western Blot in WI38 whole cell lysate. This antibody also gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
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常规说明
This antibody clone is manufactured by Abcam.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度Protein G purified
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克隆单克隆
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克隆编号5A6
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同种型IgG2b
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab28320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. | |
| WB | Use a concentration of 1 µg/ml. Use under non reducing condition. Detects a band of approximately 80 kDa (predicted molecular weight: 85 kDa). Abcam do not recommend adding a reducing agent (e.g. DTT) to lysates or heating samples prior to loading on SDS-PAGE gels. We have used BSA as our blocking agent at 1%. |
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| IHC-P | Use a concentration of 10 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
靶标
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功能Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
组织特异性Most abundant in liver, brain and lung.
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序列相似性Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
翻译后修饰Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
细胞定位Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
- Information by UniProt
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数据库链接
- Entrez Gene: 4035 Human
- Entrez Gene: 16971 Mouse
- Entrez Gene: 100009547 Rabbit
- Entrez Gene: 299858 Rat
- Omim: 107770 Human
- SwissProt: Q07954 Human
- SwissProt: Q91ZX7 Mouse
- Unigene: 162757 Human
see all -
别名
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
Anti-LRP1 antibody [5A6] 图像
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![Western blot - Anti-LRP1 antibody [5A6] (ab28320)](http://www.abcam.cn/ps/products/28/ab28320/Images/ab28320-171774-ab28320AP1253050.jpg)
Western blot - Anti-LRP1 antibody [5A6] (ab28320)Anti-LRP1 antibody [5A6] (ab28320) at 1 µg/ml + WI38 (Human lung fibroblast cell line) Whole Cell Lysate [Non-reduced] at 25 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
Developed using the ECL technique
Predicted band size : 85 kDa
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Bovine Serum Albumin before being incubated with ab28320 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution. Please note that no reducing agent was added to the cell lysate and the sample was not heated prior to loading onto the SDS-PAGE gel.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [5A6] (ab28320)](http://www.abcam.cn/ps/products/28/ab28320/Images/LRP1-Primary-antibodies-ab28320-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP1 antibody [5A6] (ab28320)IHC image of LRP1 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28320, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry - Low Density LRP antibody [5A6] (ab28320)](http://www.abcam.cn/ps/products/28/ab28320/Images/Low-Density-LRP-Primary-antibodies-ab28320-1.jpg)
Flow Cytometry - Low Density LRP antibody [5A6] (ab28320)Overlay histogram showing HepG2 cells stained with ab28320 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28320, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunocytochemistry/ Immunofluorescence - Low Density LRP antibody [5A6] (ab28320)](http://www.abcam.cn/ps/products/28/ab28320/Images/ab28320_1.jpg)
Immunocytochemistry/ Immunofluorescence - Low Density LRP antibody [5A6] (ab28320)This image is courtesy of an anonymous Abreviewab28320 at 1/100 staining human HUVEC cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incuabtion with the antibody for 1 hour. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary. -
![Western blot - Low Density LRP antibody [5A6] (ab28320)](http://www.abcam.cn/ps/products/28/ab28320/Images/ab28320_2.jpg)
Western blot - Low Density LRP antibody [5A6] (ab28320)This image is courtesy of an Abreview submitted by Dr Torry Tuckerab28320 at 1/3000 + WI38 fibroblasts whole cell lysate
Secondary
HRP conjugated donkey anti-mouse polyclonal diluted 1/10000
Developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 2 minutesThis image is courtesy of an Abreview submitted by Dr Torry Tucker
50µg of human fibroblast whole cell lysate were loaded on to the gel and run under denaturing conditions.
The membrane was blocked with 1% BSA for 1 hour at RT.
The primary antibody was incubated for 12 hours at 4°C.
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![Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [5A6] (ab28320)](http://www.abcam.cn/ps/products/28/ab28320/Images/LRP1-Primary-antibodies-ab28320-127.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [5A6] (ab28320)This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United KingdomICC/IF image of LRP1 staining in rat mixed glia culture using ab28320 (1:500). The cells were fixed uisng paraformaldehyde and permeabilised using 0.3% TritonX in 0.1%PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. the Cells were then inubated with ab23020 for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to anti mouse IgG conjuagted to Alexa Fluor 568.
Anti-LRP1 antibody [5A6] (ab28320)参考文献
This product has been referenced in:
- Wang X et al. Improvement of Electroacupuncture on APP/PS1 Transgenic Mice in Spatial Learning and Memory Probably due to Expression of Aß and LRP1 in Hippocampus. Evid Based Complement Alternat Med 2016:7603975 (2016). Mouse . Read more (PubMed: 27829865) »
- Zhou GL et al. Deficits in receptor-mediated endocytosis and recycling in cells from mice with Gpr107 locus disruption. J Cell Sci 127:3916-27 (2014). Read more (PubMed: 24849652) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"