Anti-LRG1抗体(ab63836)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to LRG1
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LRG1.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total protein; Human Spleen; Human Small Intestine; Human Lung. IHC-P: FFPE human liver tissue sections.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 38 kDa).

靶标

  • 组织特异性
    Plasma.
  • 序列相似性
    Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1300008B03Rik antibody
    • 2310031E04Rik antibody
    • A2GL antibody
    • A2GL_HUMAN antibody
    • HMFT1766 antibody
    • Leucine rich alpha 2 glycoprotein antibody
    • Leucine-rich alpha-2-glycoprotein 1 antibody
    • Leucine-rich alpha-2-glycoprotein antibody
    • Leucine-rich alpha-2-glycoprotein precursor antibody
    • LRG antibody
    • LRG1 antibody
    see all

图片

  • IHC image of LRG1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63836, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-LRG1 antibody (ab63836) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human Plasma Total Protein Lysate
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 secondsLRG1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

文献

ab63836 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your reply.

I have had a look at all the anti-LRG1 antibodies we have to see if any of them might be able to pick up this difference in glycosylation.

The only one I would suggest might be worth trying is the ab63836. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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