antigen sequence, corresponding to internal sequence amino acids 310-421 of Human LRCH1.
Human hippocampus tissue; RT 4, U 251 MG, Human Liver, and Human Tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 CH (calponin-homology) domain.
Contains 9 LRR (leucine-rich) repeats.
Information by UniProt
Calponin homology domain-containing protein 1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-LRCH1 antibody (ab121679)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, nucleoli, cytoplasm and cytoskeleton (actin filaments). Recommended concentration of ab121679 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRCH1 antibody (ab121679)
ab121679 at 1/400 dilution staining LRCH1 in Paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
Western blot - Anti-LRCH1 antibody (ab121679)
All lanes : Anti-LRCH1 antibody (ab121679) at 1/250 dilution Lane 1 : RT 4 lysate Lane 2 : U 251 MG lysate Lane 3 : Human plasma lysate Lane 4 : Human Liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"