Anti-LOXL4抗体(ab88186)
Key features and details
- Rabbit polyclonal to LOXL4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LOXL4抗体
参阅全部 LOXL4 一抗 -
描述
兔多克隆抗体to LOXL4 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: FFPE human testis tissue sections
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88186于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
May modulate the formation of a collagenous extracellular matrix. -
组织特异性
Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage. -
序列相似性
Belongs to the lysyl oxidase family.
Contains 4 SRCR domains. -
翻译后修饰
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 84171 Human
- Omim: 607318 Human
- SwissProt: Q96JB6 Human
- Unigene: 306814 Human
- Unigene: 671890 Human
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别名
- 4833426I20Rik antibody
- FLJ21889 antibody
- LOX L4 antibody
see all
图片
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IHC image of LOXL4 staining in a section of formalin-fixed paraffin-embedded human normal testis* performed on aLeica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab88186, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab88186 被引用在 7 文献中.
- Tan J et al. Identification and Analysis of Three Hub Prognostic Genes Related to Osteosarcoma Metastasis. J Oncol 2021:6646459 (2021). PubMed: 33564309
- Yin H et al. EZH2-mediated Epigenetic Silencing of miR-29/miR-30 targets LOXL4 and contributes to Tumorigenesis, Metastasis, and Immune Microenvironment Remodeling in Breast Cancer. Theranostics 10:8494-8512 (2020). PubMed: 32754259
- Li R et al. Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis. Mol Cancer 18:18 (2019). PubMed: 30704479
- Xie W et al. Clinical significance of LOXL4 expression and features of LOXL4-associated protein-protein interaction network in esophageal squamous cell carcinoma. Amino Acids 51:813-828 (2019). PubMed: 30900087
- Terajima M et al. Cyclophilin B control of lysine post-translational modifications of skin type I collagen. PLoS Genet 15:e1008196 (2019). PubMed: 31173582
- Comptour A et al. Lysyl oxidase-like 4 involvement in retinoic acid epithelial wound healing. Sci Rep 6:32688 (2016). PubMed: 27597564
- Jiang WP et al. Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis. Int J Oral Sci 6:31-8 (2014). WB, IHC-P ; Human . PubMed: 24357854