概述

  • 产品名称Anti-LOXL2抗体
    参阅全部 LOXL2 一抗
  • 描述
    兔多克隆抗体to LOXL2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 454-712 of Human LOXL2 (NP_002309).

  • 阳性对照
    • 293T, A431 and H1299 whole cell lysates; HeLa, HepG2, Molt-4 and Raji lysates

性能

应用

Our Abpromise guarantee covers the use of ab96233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 组织特异性Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • 序列相似性Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • 翻译后修饰The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Anti-LOXL2 antibody 图像

  • All lanes : Anti-LOXL2 antibody (ab96233) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : H1299 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 87 kDa
  • IHC image of ab96233 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96233, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-LOXL2 antibody (ab96233)参考文献

This product has been referenced in:
  • Mammoto A  et al. Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics. Nat Commun 4:1759 (2013). Read more (PubMed: 23612300) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Other
Sample Human Tissue sections (human cartilage)
Specification human cartilage
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
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提交于 Apr 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0, microwave 65c
Sample Mouse Tissue sections (mouse knee joint)
Specification mouse knee joint
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 18 2014

These were lysates of non-transfected cells, so the antibody is detecting native LOXL2.

Thank you for your patience. IgG ab27472 LOT: GR49002-1 I can confirm that the concentration is 2 ug/ml. Please do not hesitate to contact me with additional questions or concerns.

Thank you for your enquiry and your interest. s-SMA ab15734 LOT: GR17115-1 concentration: 0.3 mg/ml LOX ab31238 LOT: GR13790-1 concentration: 1 mg/ml LOXL2 ab96233 LOT: GR6538-6 concentration: 1 mg/ml I am contacting the Lab to find out the...

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Thank you for your enquiry and interest. Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should mat...

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