概述

  • 产品名称Anti-LOXL2抗体
    参阅全部 LOXL2 一抗
  • 描述
    小鼠单克隆抗体to LOXL2
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment with tag:

    NFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESD YSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSP

    corresponding to amino acids 675-774 of Human LOXL2

性能

应用

Our Abpromise guarantee covers the use of ab55470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 86 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen and transfected lysates. We have no data on the detection of endogenous protein.
ChIP Use at an assay dependent concentration.

靶标

  • 组织特异性Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • 序列相似性Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • 翻译后修饰The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Anti-LOXL2 antibody 图像



  • Predicted band size : 86 kDa
  • ChIP analysis of mouse embryonic fibroblasts using ab55470 to bind LOXL2.

    Chromatin was obtained by cross-linking with 1% formaldehyde at 37°C for 10 minutes. The primary antibody was used at 1/500 dilution for 16 hours at 4°C. Protein binding was detected using real-time PCR.

    See Abreview

Anti-LOXL2 antibody (ab55470)参考文献

This product has been referenced in:
  • Millanes-Romero A  et al. Regulation of heterochromatin transcription by Snail1/LOXL2 during epithelial-to-mesenchymal transition. Mol Cell 52:746-57 (2013). Read more (PubMed: 24239292) »

See 1 Publication for this product

Product Wall

Abreviews
Application ChIP
Sample Mouse Cell lysate - whole cell (MEFs)
Specification MEFs
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1 % (37ºC)
Detection step Real-time PCR
Positive control Experiment previously done with an unavailable antibody from ABCAM (ab60753)
Username

Abcam user community

Verified customer

提交于 Jan 21 2013

Application Western blot
Sample Human Cell lysate - whole cell (human MEFs)
Loading amount 40 µg
Specification human MEFs
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

提交于 Dec 04 2012

Gracias por tu interés en nuestro sistema Abreview.

He creado un código de descuento: XXXX para testar ab55470 en ChIP.

El código caduca el día XXXXX

Este código es canjeable por un anticu...

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Gracias por contactarnos.

Lamento mucho que el producto que os interesa haya sido descatalogado. El motivo para ello es que el colaborador que nos lo proporcionaba ha dejado de fabricarlo.

La alternativa sugerida es un buen anticuer...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (MDA MB231 WT condition media)
Total protein in input 70 µg
Specification MDA MB231 WT condition media
Immuno-precipitation step Protein G
Username

Miss. Joan Chang

Verified customer

提交于 Jun 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"