产品名称 Anti-LOXL2抗体 参阅全部 LOXL2 一抗
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Horse, Macaque Monkey, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LOXL2.
This antibody gave a positive signal in the following whole cell lysates: LNCaP; DU145; A431; A549; MDA MB 231; MCF7.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
组织特异性 Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
序列相似性 Belongs to the lysyl oxidase family. Contains 4 SRCR domains.
翻译后修饰 The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
细胞定位 Secreted > extracellular space.
Information by UniProt
LOR 2 antibody
LOX L2 antibody
Anti-LOXL2 antibody 图像
Western blot - Anti-LOXL2 antibody (ab113201)
All lanes : Anti-LOXL2 antibody (ab113201) at 1 µg/ml Lane 1 : LNCaP human prostate carcinoma cell line lysate Whole Cell Lysate Lane 2 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 86 kDa Observed band size : 90 kDa ( why is the actual band size different from the predicted?) Additional bands at : 35 kDa,86 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands. Exposure time : 150 seconds
Anti-LOXL2 antibody (ab113201)参考文献
has not yet been referenced specifically in any publications.
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