概述

  • 产品名称Anti-LOXL1抗体
    参阅全部 LOXL1 一抗
  • 描述
    兔多克隆抗体to LOXL1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cat
  • 免疫原

    Synthetic peptide: RWRQLIQWEN NGQVYSLLNS GSEYVPAGPQ RSESSSRVLL AGAPQAQQRR , corresponding to a region within N terminal amino acids 38 - 87 of Human LOXL1 (NP_005567)

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab81488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use a concentration of 5 µg/ml.

靶标

  • 相关性LOXL1 is a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL1 is active on elastin and collagen substrates. Genetic variations in LOXL1 are associated with risk of developing exfoliation syndrome (XFS) [MIM:177650]; also called exfoliation glaucoma (XFG). Exfoliation syndrome (XFS) is characterized by accumulation of abnormal microfibrillar deposits that line the aqueous bathed surfaces of the anterior segment of the eye. The prevalence of XFS increases with age, and a number of studies have pointed to a geographical clustering of XFS, although this condition is found worldwide; reported prevalence rates average about 10 to 20% of the general population over age 60.
  • 细胞定位Secreted, extracellular space.
  • 数据库链接
  • 别名
    • Lysyl oxidase like 1 antibody
    • LOL antibody
    • LOXL antibody
    • Lysyl oxidase homolog 1 antibody
    • Lysyl oxidase like protein 1 antibody
    see all

Anti-LOXL1 antibody 图像

  • Anti-LOXL1 antibody (ab81488) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
  • LOXL1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to LOXL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab81488.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 63kDa; LOXL1

Anti-LOXL1 antibody (ab81488)参考文献

This product has been referenced in:
  • Ohmura H  et al. Cardiomyocyte-specific transgenic expression of lysyl oxidase-like protein-1 induces cardiac hypertrophy in mice. Hypertens Res : (2012). WB ; Mouse . Read more (PubMed: 22763477) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEFs)
Loading amount 5 µg
Specification MEFs
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

提交于 Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 5 µg
Specification 293T
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Raul Peña

Verified customer

提交于 Feb 22 2013

Thank you for contacting Abcam. As requested please find attached the Certificate of Analysis and MSDS for: ab66339 ab81488 If there is anything else that we can help you with, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"