Anti-LOXL1抗体(ab70190)

概述

  • 产品名称Anti-LOXL1抗体
    参阅全部 LOXL1 一抗
  • 描述
    小鼠多克隆抗体to LOXL1
  • 特异性This antibody is reactive against mammalian transfected lysate.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    LOXL1 (NP_005567.2, 1 a.a. ~ 574 a.a) full-length human protein.

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab70190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

靶标

  • 相关性LOXL1 is a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL1 is active on elastin and collagen substrates. Genetic variations in LOXL1 are associated with risk of developing exfoliation syndrome (XFS) [MIM:177650]; also called exfoliation glaucoma (XFG). Exfoliation syndrome (XFS) is characterized by accumulation of abnormal microfibrillar deposits that line the aqueous bathed surfaces of the anterior segment of the eye. The prevalence of XFS increases with age, and a number of studies have pointed to a geographical clustering of XFS, although this condition is found worldwide; reported prevalence rates average about 10 to 20% of the general population over age 60.
  • 细胞定位Secreted, extracellular space.
  • 数据库链接
  • 别名
    • Lysyl oxidase like 1 antibody
    • LOL antibody
    • LOXL antibody
    • Lysyl oxidase homolog 1 antibody
    • Lysyl oxidase like protein 1 antibody
    see all

Anti-LOXL1 antibody 图像

  • All lanes : Anti-LOXL1 antibody (ab70190) at 1/500 dilution

    Lane 1 : LOXL1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-LOXL1 antibody (ab70190)参考文献

ab70190 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 5 µg
Specification 293T
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Mar 13 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEFs)
Loading amount 5 µg
Specification MEFs
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 13 2013

Gracias por contactarnos.

El código de descuento es XXXXXX, con fecha de caducidad 15- Abril-2013 y es válido para testar el anticuerpo ab70190 en lisados murinos mediante WB.

Este código tiene un valor de &euro...

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Thank you for contacting us.

The ab70190 has only been tested on the transfected LOXL1 by us. We do not have tested this antibody on the endogeneous expressed LOXL1.

The ab81488 has been tested on endogenous LOXL1 (HepG2 cells) as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"