概述

  • 产品名称
  • 描述
    兔多克隆抗体to LOX
  • 经测试应用
    适用于: WB, Flow Cyt, IHC-P, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Dog
  • 免疫原

    Synthetic peptide derived from within residues 350 to the C-terminus of Human LOX.

    (Peptide available as ab28612.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: MDA-MB-361, MDA-MB-231, MCF-7, and MEF1 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab31238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human LOX peptide (ab28612).

Abcam recommends using Milk as the blocking agent.

Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100. PubMed: 22685574
IHC-FoFr 1/300.

靶标

  • 功能
    Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
  • 组织特异性
    Heart, placenta, skeletal muscle, kidney, lung and pancreas.
  • 疾病相关
    Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
  • 序列相似性
    Belongs to the lysyl oxidase family.
  • 翻译后修饰
    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • 细胞定位
    Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • lox antibody
    • LYOX antibody
    • LYOX_HUMAN antibody
    • Lysyl oxidase antibody
    • MGC105112 antibody
    • Protein lysine 6 oxidase antibody
    • Protein-lysine 6-oxidase antibody
    see all

图片

  • ab31238 staining LOX in human saphenous vein tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween 20 and blocked with 15% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 10% serum in TBS + 0.05% Tween) for 14 hours at 4°C. An Alexa Fluor® 546-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Lanes 1 - 4 : Anti-LOX antibody (ab31238) at 1 µg/ml (Milk blocking)
    Lanes 5 - 8 : Anti-LOX antibody (ab31238) at 1 µg/ml (BSA blocking)

    Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 8 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk (lanes 1-4) or 2% Bovine Serum Albumin (lanes 5-8) before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Anti-LOX antibody (ab31238) at 1 µg/ml + MDA-MB-361 whole cell lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab31238 staining LOX in adult rat cardiac fibroblast cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100. The sample was incubated with the primary antibody (1µg/cells in PBS + BSA) for 45 minutes at 4°C. A FITC-conjugated goat anti-rabbit IgG (1/160) was used as the secondary antibody.
    Gating Strategy: 10000 cells.

    See Abreview

  • ab31238 staining LOX in mouse 15.5dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS in TBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-LOX antibody (ab31238) at 1 µg/ml

    Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Additional bands at : 15 kDa,36 kDa (possible mature (processed) protein),47 kDa (possible immature (unprocessed)),52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemical analysis of murine embryonic spinal tissue, staining LOX with ab31238.

    Tissue was permeabilized with 0.025% Triton-X and blocked for nonspecific binding using blocking solution. Sections were incubated overnight with primary antibody (1/100) and staining was detected using DAB.

文献

This product has been referenced in:
  • Comunanza V  et al. VEGF blockade enhances the antitumor effect of BRAFV600E inhibition. EMBO Mol Med 9:219-237 (2017). IF ; Human . Read more (PubMed: 27974353) »
  • Fraga A  et al. Genetic polymorphisms in key hypoxia-regulated downstream molecules and phenotypic correlation in prostate cancer. BMC Urol 17:12 (2017). Read more (PubMed: 28143503) »

See all 24 Publications for this product

客户评价及客户问答

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am atta...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Heart)
Permeabilization
Yes - 0,2% Triton
Specification
Heart
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 28°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 23 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (Secreted media from LOX-overexpressing cells)
Total protein in input
200 µg
Treatment
Control and treated with protease X
Immuno-precipitation step
Protein A/G
Specification
Secreted media from LOX-overexpressing cells
Username

Dr. Fernando Rodrguez-Pascual

Verified customer

提交于 Sep 27 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Renal tubular primary cell culture)
Permeabilization
Yes - Triton
Specification
Renal tubular primary cell culture
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Barbara Torsello

Verified customer

提交于 Mar 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Heart)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinas K - Ready to use (Dako - S3020)
Permeabilization
No
Specification
Heart
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Adipose tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate ph6
Permeabilization
Yes - Tween 1%
Specification
Adipose tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 20 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (white adipose tissue)
Gel Running Conditions
Reduced Denaturing (7%)
Loading amount
20 µg
Treatment
control and high fat diet-fed rats
Specification
white adipose tissue
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1000µg/mL · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 16 2015

Application
Western blot
Sample
Human Cell lysate - other (VSMC supernatant)
Gel Running Conditions
Non-reduced Denaturing (7%)
Loading amount
20 µg
Specification
VSMC supernatant
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1000µg/mL · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 28 2015

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 0,3% Triton X-100
Sample
Rat Cell (adult Cardiac Fibroblast)
Specification
adult Cardiac Fibroblast
Gating Strategy
10000 cells
Preparation
Cell harvesting/tissue preparation method: Trip-EDTA
Sample buffer: PBS-BSA
Username

Abcam user community

Verified customer

提交于 Jul 18 2014

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