Anti-LOX 1抗体(ab60178)
Key features and details
- Rabbit polyclonal to LOX 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LOX 1抗体
参阅全部 LOX 1 一抗 -
描述
兔多克隆抗体to LOX 1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human LOX 1 (N terminal).
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阳性对照
- WB Jurkat cells IHC-P: Human small intestines
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3659R LOX-1 Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60178于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (2) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa. Proform detected at 50 kDa, mature form detected at 31 kDa.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa. Proform detected at 50 kDa, mature form detected at 31 kDa. |
靶标
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功能
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
组织特异性
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
疾病相关
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
序列相似性
Contains 1 C-type lectin domain. -
结构域
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
翻译后修饰
The intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
细胞定位
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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数据库链接
- Entrez Gene: 4973 Human
- Omim: 602601 Human
- SwissProt: P78380 Human
- Unigene: 412484 Human
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别名
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
see all
图片
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All lanes : Anti-LOX 1 antibody (ab60178) at 0.5 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 31 kDa
Observed band size: 31,50 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of formalin fixed, paraffin-embedded human small intestine vessels labelling LOX 1 with ab60178 at 5 µg/ml, followed by biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Heat-induced antigen retrieval.
数据表及文件
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SDS download
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Datasheet download
文献 (61)
ab60178 被引用在 61 文献中.
- Liu H et al. Gypenoside L and Gypenoside LI Inhibit Proliferation in Renal Cell Carcinoma via Regulation of the MAPK and Arachidonic Acid Metabolism Pathways. Front Pharmacol 13:820639 (2022). PubMed: 35370678
- Tsumita T et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. Int J Cancer 151:944-956 (2022). PubMed: 35608341
- Rosa CM et al. Effects of the SGLT2 Inhibition on Cardiac Remodeling in Streptozotocin-Induced Diabetic Rats, a Model of Type 1 Diabetes Mellitus. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624845
- Wei X et al. Pharmacological inhibition of EZH2 by GSK126 decreases atherosclerosis by modulating foam cell formation and monocyte adhesion in apolipoprotein E-deficient mice. Exp Ther Med 22:841 (2021). PubMed: 34149887
- Murdocca M et al. Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34439368