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Our Abpromise guarantee covers the use of ab60178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with LOX 1 peptide (ab183565). Proform detected at 50 kDa, mature form detected at 31 kDa.|
ab60178 staining LOX 1 in mouse heart and carotid artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody. Counter stained with DAPI.