Anti-LONRF3抗体(ab57736)

概述

  • 产品名称Anti-LONRF3抗体
    参阅全部 LONRF3 一抗
  • 描述
    小鼠单克隆抗体to LONRF3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MESVRIEQML SLPAEVSSDN LESAERGASA AQVDMGPHPK VAAEGPAPLP TREPEQEQSP GTSTPESKVL LTQADALASR GRIREALEVY , corresponding to amino acids 1-91 of Human LONRF3

性能

应用

Our Abpromise guarantee covers the use of ab57736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Contains 1 Lon domain.
      Contains 2 RING-type zinc fingers.
      Contains 4 TPR repeats.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ22612 antibody
      • LON peptidase N terminal domain and ring finger 3 antibody
      • LON peptidase N terminal domain and ring finger protein 3 antibody
      • LON peptidase N-terminal domain and RING finger protein 3 antibody
      • LONF3_HUMAN antibody
      • LONRF3 antibody
      • MGC119463 antibody
      • MGC119465 antibody
      • OTTHUMP00000023893 antibody
      • RING finger protein 127 antibody
      • RNF127 antibody
      see all

    Anti-LONRF3 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab57736 LONRF3 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
    • All lanes : Anti-LONRF3 antibody (ab57736) at 1/1000 dilution

      Lane 1 : Whole cell lysate of SF21 + Virus infection, expressing LONRF3
      Lane 2 : Whole cell lysate of SF21 - Virus infection, expressing LONRF3

      Lysates/proteins at 40 µg per lane.

      Secondary
      IRDye 800CW conjugated mouse anti mouse antibody
      Developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an Abreview submitted by Mr Michael Dreiseidler

      See Abreview

    Anti-LONRF3 antibody (ab57736)参考文献

    ab57736 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (SF21)
    Loading amount 40 µg
    Specification SF21
    Treatment Virus infection, expressing LONRF3
    Gel Running Conditions Reduced Denaturing (12,5)
    Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Mr. Jörg Hoehfeld

    Verified customer

    提交于 Mar 14 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"