概述

  • 产品名称Anti-LOK抗体
    参阅全部 LOK 一抗
  • 描述
    兔多克隆抗体to LOK
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 900-968 of Human LOK

  • 阳性对照
    • Whole cell lysate from HeLa cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab70484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/15000. Predicted molecular weight: 112 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
    • 组织特异性Expressed predominantly in lymphoid organs.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LOK antibody
      • Lymphocyte oriented kinase antibody
      • Lymphocyte-oriented kinase antibody
      • PRO2729 antibody
      • Serine threonine protein kinase 10 antibody
      • Serine/threonine-protein kinase 10 antibody
      • STK10 antibody
      • STK10_HUMAN antibody
      see all

    Anti-LOK antibody 图像

    • All lanes : Anti-LOK antibody (ab70484) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 112 kDa
    • Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
      Lane 1: input of Human LOK at 50µg
      Lane 2: Other anti-human LOK antibody
      Lane 3: Other anti-human LOK antibody plus blocking peptide
      Lane 4: ab70484
      Lane 5: ab70484 plus blocking peptide
      Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
      Chemiluminescence with exposure times of 1 to 2 minutes.

    Anti-LOK antibody (ab70484)参考文献

    ab70484 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70484.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"