We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Synthetic peptide, corresponding to a region between amino acids 350-400 of Human LOK
Whole cell lysate from HeLa cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 1-4µg/mg of lysate.
WB: 1/2000 - 1/15000. Predicted molecular weight: 112 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
Expressed predominantly in lymphoid organs.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Information by UniProt
Lymphocyte oriented kinase antibody
Lymphocyte-oriented kinase antibody
Western blot - LOK antibody (ab70483)
All lanes : Anti-LOK antibody (ab70483) at 0.1 µg/ml Lane 1 : Whole cell lysate from HeLa cells at 5 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 50 µg Developed using the ECL technique Predicted band size : 112 kDa Observed band size : 112 kDa
Immunoprecipitation - LOK antibody (ab70483)
Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70483 at 1 µg/mg lysate for IP.
Lane 1: input of Human LOK at 50µg
Lane 2: ab70483 at 1 µg/mg lysate
Chemiluminescence with exposure times of 1 to 2 minutes.
has not yet been referenced specifically in any publications.
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