Anti-LOK抗体(ab70483)
Key features and details
- Rabbit polyclonal to LOK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LOK抗体
参阅全部 LOK 一抗 -
描述
兔多克隆抗体to LOK -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee -
免疫原
Synthetic peptide, corresponding to a region between amino acids 350-400 of Human LOK
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阳性对照
- Whole cell lysate from HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/15000. Predicted molecular weight: 112 kDa.
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IP |
Use at 1-4 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/15000. Predicted molecular weight: 112 kDa. |
IP
Use at 1-4 µg/mg of lysate. |
靶标
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功能
Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues. -
组织特异性
Expressed predominantly in lymphoid organs. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. - Information by UniProt
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数据库链接
- Entrez Gene: 6793 Human
- Omim: 603919 Human
- SwissProt: O94804 Human
- Unigene: 519756 Human
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别名
- LOK antibody
- Lymphocyte oriented kinase antibody
- Lymphocyte-oriented kinase antibody
see all
图片
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All lanes : Anti-LOK antibody (ab70483) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 112 kDa
Chemiluminescence with exposure times of 1 to 2 minutes. -
Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70483 at 1 µg/mg lysate for IP.
Lane 1: input of Human LOK at 50µg
Lane 2: ab70483 at 1 µg/mg lysate
Chemiluminescence with exposure times of 1 to 2 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab70483 尚未被引用在任何文献中。