概述

  • 产品名称
  • 描述
    兔多克隆抗体to LOK
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human LOK aa 692-721 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O94804

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human hepatocarcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 112 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
  • 组织特异性
    Expressed predominantly in lymphoid organs.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LOK antibody
    • Lymphocyte oriented kinase antibody
    • Lymphocyte-oriented kinase antibody
    • PRO2729 antibody
    • Serine threonine protein kinase 10 antibody
    • Serine/threonine-protein kinase 10 antibody
    • STK10 antibody
    • STK10_HUMAN antibody
    see all

图片

  • Anti-LOK antibody (ab37727) + Jurkat cell lysate

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling LOK with ab37727. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

文献

ab37727 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblast cells +/+ and +/- Stk10)
Loading amount
25 µg
Specification
lymphoblast cells +/+ and +/- Stk10
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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提交于 Feb 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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