The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 21 kDa). Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3 kDa according to NP_001078920.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Note=A chromosomal aberration involving LNP1 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with NUP98.