Anti-LMX1A抗体- C-terminal (ab139726)
Key features and details
- Rabbit polyclonal to LMX1A - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-LMX1A抗体- C-terminal
参阅全部 LMX1A 一抗 -
描述
兔多克隆抗体to LMX1A - C-terminal -
宿主
Rabbit -
特异性
At least two isoforms are known to exist. ab139726 is predicted to not cross-react with LMX1B. -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human LMX1A aa 300-350 (C terminal). The exact sequence is proprietary. 16 amino acids. NP_796372
Database link: NP_796372 -
阳性对照
- WB: Rat brain tissue lysate. IHC-P: Mouse and human brain tissue. ICC/IF: Mouse neural stem cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab139726于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
Only validated in rat samples. |
|
ICC/IF | (1) |
Use a concentration of 20 µg/ml.
|
IHC-P |
Use a concentration of 2.5 µg/ml.
|
说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa. Only validated in rat samples. |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae. -
组织特异性
Isoform 1 is expressed in many tissues. Not found in heart, liver, spleen and testis. Relatively highly expressed in fetal brain. Isoform LMX1A-4B is expressed in testis. -
序列相似性
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4009 Human
- Entrez Gene: 110648 Mouse
- Entrez Gene: 289201 Rat
- GenBank: AY078391.1 Human
- Omim: 600298 Human
- SwissProt: Q8TE12 Human
- SwissProt: Q9JKU8 Mouse
- Unigene: 667312 Human
see all -
别名
- HGNC:6653 antibody
- LIM homeobox protein 1.1 antibody
- LIM homeobox transcription factor 1 alpha antibody
see all
图片
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ab139726 staining LMX1A (red) in mouse neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 3% BSA + 0.3% Triton X-100) for 16 hours at 4°C. An Alexa-Fluor® 568-conjugated donkey anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody.
Blue - DAPI, Green - Nestin.
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Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling LMX1A with ab139726 at 5 μg/ml.
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Lane 1 : Anti-LMX1A antibody - C-terminal (ab139726) at 1 µg
Lane 2 : Anti-LMX1A antibody - C-terminal (ab139726) at 2 µg
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling LMX1A with antibody ab139726 at 2.5 μg/ml.
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Immunofluorescence of human brain tissue labeling LMX1A with ab139726 at 20 μg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab139726 被引用在 11 文献中.
- Novak G et al. Single-cell transcriptomics of human iPSC differentiation dynamics reveal a core molecular network of Parkinson's disease. Commun Biol 5:49 (2022). PubMed: 35027645
- Zhang J et al. Effect of NTN and Lmx1α on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells. Parkinsons Dis 2021:6676709 (2021). PubMed: 34373779
- Ahmed M et al. The chromatin remodelling factor Chd7 protects auditory neurons and sensory hair cells from stress-induced degeneration. Commun Biol 4:1260 (2021). PubMed: 34732824
- Renner H et al. A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids. Elife 9:N/A (2020). PubMed: 33138918
- Candelario KM et al. Exosome/microvesicle content is altered in leucine-rich repeat kinase 2 mutant induced pluripotent stem cell-derived neural cells. J Comp Neurol 528:1203-1215 (2020). PubMed: 31743443