Anti-LMTK3抗体(ab110516)

概述

  • 产品名称Anti-LMTK3抗体
    参阅全部 LMTK3 一抗
  • 描述
    兔多克隆抗体to LMTK3
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 320-350 of Human LMTK3 (XP_055866.6).

  • 阳性对照
    • Mouse liver tissue lysates. IHC-P: Mouse liver FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab110516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 153 kDa.

靶标

Anti-LMTK3 antibody 图像

  • IHC image of LMTK3 staining in mouse liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110516, 10µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-LMTK3 antibody (ab110516) at 1/100 dilution + Mouse liver tissue lysates at 35 µg

    Predicted band size : 153 kDa

Anti-LMTK3 antibody (ab110516)参考文献

ab110516 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us, I am sorry that you are experiencing some problem with ab110516. There is no particular protocol we recommendfor this antibody, so I think a standard procedure would work. However, please find below some troubleshooting tip...

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Thank you for your inquiry. Both antibodies recognize the target protein you are looking for: LMTK3. Which one is most suitable for your purposes depends on the species you are probing for and if you would like to probe the WB multiple times ( an...

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