The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
Contains 2 LIM zinc-binding domains.
The second LIM zinc-binding domain interacts with KDM5A.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMO2 antibody [1A9-3D11] (ab64139)This image is courtesy of an anonymous abreview.
ab64139 staining LMO2 in human lymph node tissue section by Immunohistochemistry(Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, antigen retrieval in Target Retrieval Solution and blocking in 100% serum for 10 minutes at 25°C. The primary antibody was diluted, 1/700 and incubated with sample for 1 hour at 25°C. A polymer linked mouse polyclonal to mouse IgG1 was used undiluted, as secondary.
This product has been referenced in:
Funk KE et al. Distinct Therapeutic Mechanisms of Tau Antibodies: Promoting Microglial Clearance Versus Blocking Neuronal Uptake. J Biol Chem290:21652-62 (2015).
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